Journal: International Journal of Oral Science

Article Title: An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements

doi: 10.1038/ijos.2014.5

Figure Lengend Snippet: Comparing the IL-1β expression of DP cells treated with or without LPS (200 μg⋅L −1 ) for 12 and 24 h. * P <0.05, compared with DMEM. # P <0.05, compared with 12 h. DMEM, Dulbecco's modified Eagle's medium; DP, dental pulp; IL, interleukin; LPS, lipopolysaccharide; OD, optical density.

Article Snippet: These protein samples (25 μg) were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes and incubated with primary antibodies against IL-1β (GTX22105, 1∶3 000; GeneTex, San Antonio, TX, USA) and β-actin (GTX300041, 1∶5 000; GeneTex, San Antonio, TX, USA).

Techniques: Expressing, Modification

Journal: International Journal of Oral Science

Article Title: An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements

doi: 10.1038/ijos.2014.5

Figure Lengend Snippet: IL-1β expression of LPS-treated DP cells cultured on MTA or CS cements. * P <0.05, compared with MTA. CS, calcium silicate; DP, dental pulp; LPS, lipopolysaccharide; MTA, mineral trioxide aggregate.

Article Snippet: These protein samples (25 μg) were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes and incubated with primary antibodies against IL-1β (GTX22105, 1∶3 000; GeneTex, San Antonio, TX, USA) and β-actin (GTX300041, 1∶5 000; GeneTex, San Antonio, TX, USA).

Techniques: Expressing, Cell Culture

Journal: Annals of Translational Medicine

Article Title: Neuroprotective effects of novel compound Tozan on cognition, neurogenesis and apoptosis in diabetes

doi: 10.21037/atm-21-4439

Figure Lengend Snippet: Effects of Tozan on the anti-apoptotic and nerve regeneration effects in the MG-induced ERβ knockdown SH-SY5Y cells. (A,B) The protein expression of apoptosis-related proteins Bcl-2, Bax, procaspase-3, and cleaved caspase-3 were detected by western blot. (C,D) The quantification of Bcl-2/Bax and cleaved caspase-3/procaspase-3 is shown as the ratio (in percentage) of the Veh + Veh group. Data are shown as means ± SEM (n=3). **P<0.01 vs. LV-ERβ-RNAi + MG group. (E) Effects of Tozan on the number of nascent cells in MG-induced ERβ knockdown SH-SY5Y cells using Immunofluorescence staining. Representative images of BrdU-labeled nascent cells of all groups. Scale bars =100 µM. (F) The results are shown as the ratio of BrdU-labeled cells to DAPI-labeled cells (in percentage) of the Veh + Veh group. Data are shown as means ± SEM (n=3). **P<0.01 vs. LV-ERβ-RNAi + MG group. (G) Effects of Tozan on the expression of BDNF protein in the MG-induced ERβ knockdown SH-SY5Y cells. The protein expression of BDNF in all groups was detected by western blot. (H) Quantitative analysis results of BDNF are shown as the ratio (in percentage) of the Veh + Veh group. Data are shown as means ± SEM (n=3). **P<0.01 vs. LV-ERβ-RNAi + MG group. MG, methylglyoxal; ER, estrogen receptor; SEM, standard error of mean.

Article Snippet: The membranes were then incubated with the respective primary antibodies against BDNF (1:1,000, Cat. No. BS6533, Bioworld Technology), pro- or cleaved caspase-3 (1:500, Cat. No. 9665S, Cell Signaling Technology), Bcl-2 (1:500, Cat. No. BS1031, Boster Technology), Bax (1:500, Cat. No. BS1030, Boster Technology), ERα (1:1,000, Cat. No. SC-787, Santa Cruz Biotechnology), ERβ (1:1,000, Cat. No. SC-8974, Santa Cruz Biotechnology) GAPDH (1:8,000, Cat. No. AP0063, Bioworld Technology) or β-actin (1:10,000, Cat. No. AP0060, Bioworld Technology) at 4 °C overnight.

Techniques: Knockdown, Expressing, Western Blot, Immunofluorescence, Staining, Labeling